Maize LEC1 Improves Transformation in Both Maize and Wheat
AbstractSince ectopic expression of LEC1 in Arabidopsis can lead to adventive formation of embryo-like structures (Lotan et al., 1998), it was hypothesized that ectopic expression of the maize LEC1...
View ArticleUsing Genes that Stimulate the Cell Cycle to Improve Maize Transformation
AbstractThe cell cycle’s impact on plant transformation has been investigated by various groups, showing that S-phase (Villemont et al., 1997), M-phase (Okada et al., 1986) or both (Meyer et al., 1985)...
View ArticleRapid genotype “independent” Zea mays L. (maize) transformation via direct...
AbstractConstitutive expression of the Zea mays L. (maize) morphogenic transcription factors Baby Boom (Bbm) and Wuschel2 (Wus2) in maize can not only greatly increase transformation efficiency but can...
View ArticleMaize Transformation Using the Morphogenic Genes Baby Boom and Wuschel2
AbstractDespite the fact that maize transformation has been available for over 25 years, the technology has remained too specialized, labor-intensive, and inefficient to be useful for the majority of...
View ArticleUse of DoE methodology to optimize the regeneration of high-quality,...
AbstractThe maize Agrobacterium tumefaciens-mediated transformation process normally takes about 10 to 15 wk from embryo infection and co-cultivation to sending “T0” plants to the greenhouse (GH). A...
View ArticleUse of non-integrating Zm-Wus2 vectors to enhance maize transformation
AbstractThe use of Baby boom (Bbm) and Wuschel2 (Wus2) has made maize transformation more efficient across an increasingly wide range of inbreds. However, the benefits have come with the requirement of...
View ArticleGermline Transformation of Maize Following Manipulation of Chimeric Shoot...
AbstractGenetic transformation of maize has been limited to a small number of genotypes that form embryogenie tissue in culture. We have investigated whether cells in the developing shoot meristem of...
View ArticleWuschel2 enables highly efficient CRISPR/Cas-targeted genome editing during...
AbstractFor many important crops including sorghum, use of CRISPR/Cas technology is limited not only by the delivery of the gene-modification components into a plant cell, but also by the ability to...
View ArticleLeaf transformation for efficient random integration and targeted genome...
AbstractTransformation in grass species has traditionally relied on immature embryos and has therefore been limited to a few major Poaceae crops. Other transformation explants, including leaf tissue,...
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